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KMID : 0364820130490020156
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Korean Journal of Microbiology
2013 Volume.49 No. 2 p.156 ~ p.164
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Diversity and Antimicrobial Activity of Actinomycetes from Fecal Sample of Rhinoceros Beetle Larvae
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Lee Hye-Won
Ahn Jae-Hyung
Kim Min-Wook
Weon Hang-Yeon
Song Jae-Kyeong
Lee Sung-Jae
Kim Byung-Yong
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Abstract
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Actinomycetes produce diverse secondary metabolites which have the primary importance in medicine, agriculture and food production, and key to this is their ability to interact with other organisms in natural habitats. In this study, we have investigated the taxonomical and functional diversity of actinomycetes in fecal sample of rhinoceros beetle larvae (Allomyrina dichotoma L.) by using culture-dependent and -independent approaches. For the culture independent approach, the community DNA was extracted from the sample and 16S rRNA genes of actinomycetes were amplified using actinomycetes-specific PCR primers. Thirty-seven clones were classified into 15 genera and 24 species of actinomycetes. For the culture-dependent approach, 53 strains were isolated from larval feces, of which 27 isolates
were selected based on morphological characteristics. The isolates were classified into 4 genera and 14 species, and
24 isolates (89%) were identified as the genus Streptomyces. Many of the representative isolates had antimicrobial activities against plant pathogenic fungi and Gram-positive bacteria. In addition, most of the isolates (78%) showed
biochemical properties to hydrolyze cellulose and casein. The results demonstrated that diverse and valuable actinomycetes could be isolated from insect fecal samples, indicating that insect guts can be rich sources for novel
bioactive compounds.
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KEYWORD
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actinomycetes diversity, antimicrobial activity, Rhinoceros beetle larvae
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